Crystal structure of glutamine amidotransferase fromThermotoga maritima
نویسندگان
چکیده
منابع مشابه
Role of NifS in maturation of glutamine phosphoribosylpyrophosphate amidotransferase.
Glutamine phosphoribosylpyrophosphate amidotransferase from Bacillus subtilis is synthesized as an inactive precursor that requires two maturation steps: incorporation of a [4Fe-4S] center and cleavage of an 11-residue NH2-terminal propeptide. Overproduction from a multicopy plasmid in Escherichia coli leads to the formation of soluble proenzyme and mature enzyme forms as well as a small fracti...
متن کاملMutational analysis of Bacillus subtilis glutamine phosphoribosylpyrophosphate amidotransferase propeptide processing.
Glutamine phosphoribosylpyrophosphate amidotransferase from Bacillus subtilis is a member of an N-terminal nucleophile hydrolase enzyme superfamily, several of which undergo autocatalytic propeptide processing to generate the mature active enzyme. A series of mutations was analyzed to determine whether amino acid residues required for catalysis are also used for propeptide processing. Propeptid...
متن کاملPARTIAL PURIFICATION AND PROPERTIES OF L-GLUTAMINE: D-FRUCTOSE 6-P AMIDOTRANSFERASE FROM HUMAN PLACENTA
The first enzyme of the pathway for uridine diphosphate N-acetyl-D-glucosamine (UDPAG) biosynthesis i.e. L-glutamine: D-fructose 6-P amidotransferase (E.C. 2.6.1.16) was purified 52-fold from human placenta using methanol fractionation and column chromatography on DEAE-Sephadex A-50. The enzyme showed optimal activity in a broad range of pH from 5.8 to 7.8 in both phosphate and cacodylate ...
متن کاملGlutamine-binding subunit of glutamate synthase and partial reactions catalyzed by this glutamine amidotransferase.
In the course of studies on glutamine-dependent carbamyl phosphate synthetase from Aerobacter aerogenes, we purified another protein which was found to be glutamate synthase (EC 2.6.1.53). The enzyme, obtained in apparently homogeneous form (monomer molecular weight about 227,000; s(20,omega) = 17.6 S), was found to be a typical glutamine amidotransferase in that it exhibits glutaminase activit...
متن کاملCrystal structure of the dipeptide cyclo(glycyl-l-glutamine).
that produces opioid-like effects on the central nervous system.1 Cleavage of the β-endorphin C-terminal in the brain and pituitary gland yields the biologically active dipeptide glycyl-Lglutamine (Gly-L-Gln, β-endorphin 30-31), which inhibits βendorphin-induced cardiorespiratory depression.2,3 The stereospecificity of this effect is evident, since Gly-D-Gln has recently been reported to be ina...
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ژورنال
عنوان ژورنال: Proteins: Structure, Function, and Genetics
سال: 2002
ISSN: 0887-3585,1097-0134
DOI: 10.1002/prot.10161